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| Freitas,D.A.; Leclerc,S.; Miyoshi,A.; Oliveira,S.C.; Sommer,P.S.M.; Rodrigues,L.; Correa Junior,A.; Gautier,M.; Langella,P.; Azevedo,V.A.; Le Loir,Y.. |
| Lactococcus lactis, the model lactic acid bacterium, is a good candidate for heterologous protein production in both foodstuffs and the digestive tract. We attempted to produce Streptomyces tendae antifungal protein 1 (Afp1) in L. lactis with the objective of constructing a strain able to limit fungal growth. Since Afp1 activity requires disulfide bond (DSB) formation and since intracellular redox conditions are reportedly unfavorable for DSB formation in prokaryotes, Afp1 was produced as a secreted form. An inducible expression-secretion system was used to drive Afp1 secretion by L. lactis; Afp1 was fused or not with LEISSTCDA, a synthetic propeptide (LEISS) that has been described to be a secretion enhancer. Production of Afp1 alone was not achieved, but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactococcus lactis; Antifungal protein; Secretion; Streptomyces tendae antifungal protein 1. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001100004 |
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| Nonato,L.F.; Rocha-Vieira,E.; Tossige-Gomes,R.; Soares,A.A.; Soares,B.A.; Freitas,D.A.; Oliveira,M.X.; Mendonça,V.A.; Lacerda,A.C.; Massensini,A.R.; Leite,H.R.. |
| Although it is well known that physical training ameliorates brain oxidative function after injuries by enhancing the levels of neurotrophic factors and oxidative status, there is little evidence addressing the influence of exercise training itself on brain oxidative damage and data is conflicting. This study investigated the effect of well-established swimming training protocol on lipid peroxidation and components of antioxidant system in the rat brain. Male Wistar rats were randomized into trained (5 days/week, 8 weeks, 30 min; n=8) and non-trained (n=7) groups. Forty-eight hours after the last session of exercise, animals were euthanized and the brain was collected for oxidative stress analysis. Swimming training decreased thiobarbituric acid reactive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Exercise therapy; Oxidative stress; Rat; Lipid peroxidation; Superoxide dismutase; Swimming training. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000605 |
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